The sampling requirements for Ontario Regulation 153 (O.Reg 153) PHCs are as follows:
Soil:
| Analysis | Number of Containers | Container Size | Container Type | Preservative |
| BTEX & PHCs F1 | 2 | 40 mL | Glass Vial | Methanol |
| PHCs F2-F4 | 2* | 120 mL | Clear Glass Soil Jar | No preservative |
*Note: Sample volume for moisture content can be provide in clear glass soil jar, or approved soil sampling bag.
PAHs and PCBs can also be combined with PHCs F2-F4 without requiring additional sample volume.
Water:
| Analysis | Number of Containers | Container Size | Container Type | Preservative |
| BTEX & PHCs F1 | 2 | 40 mL | Clear Glass Vial | Sodium Bisulphate |
| PHCs F2-F4* | 2 | 100 mL | Amber Glass Bottle | Sodium Bisulphate |
*Note: PAHs can be combined with PHCs F2-F4 without requiring additional sample volume.