eDNA - Frequently Asked Questions
Environmental DNA (eDNA) testing is cost effective, more accurate than traditional methods, has less risk of pathogen transfer, and is less invasive to spec...
Fri, 21 Dec, 2018 at 12:37 PM
Our current eFish eDNA assay tests for the presence of a group of 12 fish species: Sockeye salmon, Pink salmon, Chum salmon, Arctic Grayling, Cutthroat trou...
Fri, 21 Dec, 2018 at 12:39 PM
Generally, eDNA persists in the environment for a short time. The duration is dependent on environmental conditions but is quantified, in standard condition...
Fri, 21 Dec, 2018 at 12:40 PM
Suspension of eDNA in water allows for transport (active or through osmotic diffusion) through the system (transport distances are variable and depend on ou...
Fri, 21 Dec, 2018 at 12:43 PM
eDNA detects mitochondrial DNA. Thus, only the maternal lineage would be detected and subspecies cannot be identified.
Fri, 21 Dec, 2018 at 12:44 PM
Research is ongoing regarding distribution and persistence of eDNA in environments. However, at this time, there is evidence suggesting that most eDNA is fo...
Fri, 21 Dec, 2018 at 12:44 PM
The ideal volume required is 1L of water per sample which is to be filtered through a cellulose nitrate filter (45μm pore size). This increases detecti...
Fri, 21 Dec, 2018 at 12:46 PM
Water samples should be collected in 1L Nalgene bottles. If the water sample is to be filtered in the field, insert the rolled-up filter in a 2mL tube,...
Fri, 21 Dec, 2018 at 12:49 PM
No. It is not necessary to submit preserved filters on ice. Samples should be filtered prior to submission to maximize the potential DNA recovery and redu...
Fri, 21 Dec, 2018 at 12:52 PM
It depends on the contents of the water sample and what remains on the filter. Filtering the water sample and preserving the filter within 24 hours of ...
Fri, 21 Dec, 2018 at 1:30 PM